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Author Topic: Build out ideas DIYBioSpace  (Read 1901 times)

macrutan

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Build out ideas DIYBioSpace
« on: October 12, 2014, 09:07:48 PM »
     As we approach the build out, we have the need to identify the minutia of the details for each subspace. This thread is to help identify and define those details for the DIYBioSpace.
Below are some ideas to keep the conversation going?
Mac?s questions:
Architectural/construction/design considerations:
1) Has the specific layout of the DIYBioSpace been determined yet?
2) Is there a detailed blueprint for this room?s layout?
3) Do we have the necessary ?workstation modules? and appropriate space allocated with that design?
Ie. List all project and workspace scenarios we can conjure, research, assess, and then design.
What is the proposed project list?
Inspiration: I had an architectural client that built a database for every aspect of industrial health/medical buildings. From that they developed an app that calculated to the square footage each office space based on the of health/medical services provided. The data included hardware and workstation information with relative human factors and space requirements. It even calculated the parking spaces required for the building. I do not have access to the application anymore but we can use this to inspire our planned usage.
Note/Suggestion: A wall mounted countertop implementation for work surface space with no cabinets below. This will allow legroom and temporary storage of bins, hardware, etc. under the counter and optimizes the workspace.
4) What are the min/max/median workspace sizes and how many ?Seats? are practical?
5) Are there any multipurpose uses that the DIYBioSpace and the adjacent darkroom spaces could share?
Note/Suggestion: A section of the darkroom to be utilized to house long-term bioluminescent projects?
6) Are there other ?dark project? needs?
DIYBiioSpace Project Considerations
1) What is on the current project list?
? Fluid Handling Robot
https://www.kickstarter.com/projects/930368578/openpcr-open-source-biotech-on-your-desktop
? Open PCR (Fluid Handling Robot)
http://openpcr.org/
? OpenStim
http://sourceforge.net/p/openbrainstim/wiki/Safety%20Information/
2) Do we need to acquire medical-grade electrical isolation device to assure safe use practices?
I'm committed to helping design and build the space as we approach the build out as much as my schedule permits. Dave Woods and Mike King have been on top of the plans for the whole of new space.
Would any of you like to spend an hour or so meeting to mock up the layout? Can we start a list of potential stations that are essential and also start to discuss how some of these areas can be hybridized to become multifunctional and also which need to be absolutely dedicated areas?
Personally, I am particularly interested in any and all imaging technologies as well as data visualization aspects of the future DIYBioSpace. I have some long-term goals to develop embedded technologies based in this space FamiLAB and members willing.
The first of which is a versatile section of the DIYBioSpace dedicated to presentation. It would be a concentrated area for documenting and showing experiments live both in-house and online. It can be a small area approximately 4 feet of counter space that would double as workspace. I would like to spend some time at this meeting discussing this possibility with members.
Thanks for reading this epic novel,
Mac
 

da3v

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Re: Build out ideas DIYBioSpace
« Reply #1 on: October 12, 2014, 10:33:17 PM »
I just typed an equally epic reply. Where did it go?

da3v

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Re: Build out ideas DIYBioSpace
« Reply #2 on: October 12, 2014, 11:10:04 PM »
Okay, if the previous version ever reappears, please forgive this decidedly less epic second attempt:

I got a lot of good layout advice from Christine, let's get together with someone who is a better drawer-upper than I am and see what it looks like.

It seems like a good class size is 4-6 students, an instructor, and maybe a helper. A lot of workshops have time-sensitive processes that bottleneck around one piece of equipment or another, and 6 students seemed like a sane upper limit for the workshops I attended.

Partial equipment list for synthetic bio:
   laminar hood http://www.instructables.com/id/Portable-Laminar-Flow-Hood/
   centrifuge
   incubator(s)
   freezer
   microwave
   autoclave
   glassware
   place for glassware to dry
   utility sink
   gel boxes
   pipetters
   storage space
   pcr
   water bath

Partial list for algae
   tanks
   lights
   shelves
   containers
   air pumps
   more lights

partial list for plants
   same as algae, plus maybe a gene gun

For openstim/openbci/open-anything-else-involving-electrodes-on-living-things, I strongly suggest sticking to batteries, and not even trying to get AC isolation right.  Getting it wrong would really suck.

Imaging:
   We are waaaay behind Noisebridge and PS1 on the SEM front.
   Also, there is this http://makezine.com/2011/06/02/diy-gigapixel-microscopy/
   and this http://gizmodo.com/how-scientists-hacked-a-normal-microscope-into-a-gigapi-960623388
   And of course, this: http://www.instructables.com/id/A-Low-Cost-Atomic-Force-Microscope-%E4%BD%8E%E6%88%90%E6%9C%AC%E5%8E%9F%E5%AD%90%E5%8A%9B%E9%A1%AF%E5%BE%AE%E9%8F%A1/
  This could easily expand to fill the rest of the lab if I am not carefully supervised.

seating: non-absorbnet (stackable?) lab stools

Safety:
   eyewash station
   first aid kit
   fire extinguisher
   hazardous waste trash can +sharps container



I took some pictures the last time I was at GenSpace, should I post those as replies here, or is there a place that we are consolidating build-out related files?

macrutan

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Re: Build out ideas DIYBioSpace
« Reply #3 on: October 13, 2014, 01:53:34 AM »
Dave,
I'm not shocked that you prefer batteries over AC isolation.
Posting the genspace pics here should be good. It's competitive analysis. The more we know about other labs, the better we can compliment, etc.







da3v

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Re: Build out ideas DIYBioSpace
« Reply #4 on: October 14, 2014, 12:17:05 AM »
Scaled down pano looking at side of GenSpace wetlab.

da3v

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Re: Build out ideas DIYBioSpace
« Reply #5 on: October 14, 2014, 12:18:46 AM »
Door to GenSpace wetlab. Hey, what's that blue thing next to the "No Food or Drink" sign?

macrutan

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Re: Build out ideas DIYBioSpace
« Reply #6 on: October 16, 2014, 07:42:16 PM »
I like the big window factor. Also that blue thing is small but potent!

cosmicaug

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Re: Build out ideas DIYBioSpace
« Reply #7 on: August 23, 2015, 12:28:00 PM »
It probably does not add much but here's old article on making your own lab:
http://www.mlo-online.com/articles/201112/the-quest-for-the-500-home-molecular-biology-laboratory.php

cosmicaug

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Re: Build out ideas DIYBioSpace
« Reply #8 on: August 23, 2015, 12:49:04 PM »
If you ever need another gel box for agarose gels there are guides out there to MacGyver one out of common supplies. However, a well built box might provide a more uniforms electrical field (thus producing better results with less chance of smiling or frowning  gel runs) and likely be safer. It might be informative to compare the results of a jury rigged box with a commercial one  (you could even just run a dye mix for this). In any event, Familab has the tools and the skills so a well built box could probably be built in house. One could resort to cloning an existing model, creating a design from scratch or basing it on someone else's design such as the one found at http://teach.genetics.utah.edu/content/labs/build_gel_box.pdf