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Author Topic: Bio Supplies Available on Amazon  (Read 1854 times)

tetsuharu

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Bio Supplies Available on Amazon
« on: April 23, 2012, 09:49:13 AM »
I have gotten a little trigger-happy with Amazon purchases lately, and I found a bunch of bio supplies available through them. Some of the resources that are supposedly difficult to get, like the BioBlocks plates, are usually only available to universities or researchers. With Amazon I have confidence they will arrive after I hit the purchase button.


Plasmids: what do all them do? I see pUC8, but I don't know what it does, and I don't know where to find information
http://www.amazon.com/gp/search/ref=sr_nr_i_2?rh=k%3Aplasmid%2Ci%3Aindustrial&keywords=plasmid&ie=UTF8&qid=1335186994


Gel EP: there are a lot of expensive systems, but some cheap ones, too.
http://www.amazon.com/s/ref=sr_nr_n_0?rh=n%3A16310091%2Ck%3Agel+electrophoresis%2Cn%3A%2116310161%2Cn%3A317970011%2Cn%3A317980011%2Cn%3A317987011%2Cn%3A317988011&bbn=317987011&keywords=gel+electrophoresis&ie=UTF8&qid=1335187237&rnid=317987011


it's the power supplies i'm not sure about, here's one that just uses a big wall wart?
http://www.amazon.com/E-Gel-Starter-Gels-Ethidium-Bromide/dp/B005Q0GDJ6/ref=sr_1_338?s=industrial&ie=UTF8&qid=1335187494&sr=1-338


Micropipettes: I've heard they're important, but I don't know anything about buying them.


PCR Thermocyclers: There is one that I've found, it's very expensive, but only like 4x openpcr
http://www.amazon.com/Edvotek-EdvoCycler-25mL-0-2mL-Samples/dp/B004W7Y382/ref=sr_1_1?s=industrial&ie=UTF8&qid=1335187625&sr=1-1


Petri dishes are easy to find on amazon.






Thoughts?

tetsuharu

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Re: Bio Supplies Available on Amazon
« Reply #1 on: April 25, 2012, 02:53:40 AM »
Just found about about Amazon Supply:

http://www.amazonsupply.com/s/317970011/ref=gw_c_ls

willasaywhat

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Bio Supplies Available on Amazon
« Reply #2 on: April 25, 2012, 07:11:40 PM »
Amazon supply is crayzay.

comradetim

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Re: Bio Supplies Available on Amazon
« Reply #3 on: May 02, 2012, 01:07:41 PM »
Heres some info on the pUC8 plasmid:
http://www.lablife.org/p?a=vdb_view&id=g2.INMLL2zuPbe7EoyGBg90LiTG1oM-

The things in blue are restriction enzyme sites (https://en.wikipedia.org/wiki/Restriction_enzyme)
Other than that you see:
Ampicillin resistance
Lac (https://en.wikipedia.org/wiki/Lac_operon) promoter for gene expression.  You would put your gene of interest downstream of this promoter, and with proper induction, that gene would be transcribed->translated.
The thing in black is the origin of replication for copying of the plasmid within the E.coli

I'm also seeing LacZ (https://en.wikipedia.org/wiki/Beta-galactosidase), not quite sure what that is for, it might be a default expression thing if you don't put anything in the plasmid.  Could be checked with X-gal (https://en.wikipedia.org/wiki/X-gal).