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Author Topic: Ryan-Biohacking  (Read 2125 times)

Natrlselexion

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Ryan-Biohacking
« on: October 23, 2013, 06:44:08 PM »
Hi everyone,


Though a friend of a friend i got in contact with john and got a lab tour the other day.
I was really impressed with your lab, most specifically the amount of creativity that seemed to go behind everything.


A little bit about myself, Im a researcher for a pharmaceutical company, more specifically doing pre-clinical immunology research on various drug candidates


Most of my day is spent doing standard immunological assays like ELISA, ELISPOT, and Flow Cytometery. In college i did a lot of PCR (john told me about your open PCR efforts)

I would like to get into biohacking/home biology. Most standard biological instruments are actually pretty rudimentary from an electronics and engineering standpoint. I would like to get into building some of these things for general use, but don't know much about coding or hardware design.

A couple ideas i was thinking about was a water jacketed incubator, or possibly a bioreactor/fermentor using a modified version of brewpi. [size=78%]http://www.laboratory- equipment.com/gallery/Vacuumcham/incubators/Images/laboratory_incubators_water_jacketed.jpg[/size]
http://www.intechopen.com/source/html/26393/media/image6.jpg
http://brewpi.com/


Are these types of projects something y'all would be interested in? Feel free to ask me anything.


Lastly because safety always is brought up with biohacking i am well versed in the BSL (bio safety level) system. Anything in your guys space would have to conform to BSL1 standards. This includes most wet chemistry benchtop assays as well as nonpathogenic lab strand bacteria and some forms of mammalian cell culture.


Thanks!
Ryan


johnbentcope

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Re: Ryan-Biohacking
« Reply #1 on: October 23, 2013, 10:29:25 PM »

I'll vouch for this guy knowing more about safety than I do. Discussing our various biohacking events and some personal wine making attempts, he was well prepared with what precautions we took that were superfluous, and, more importantly, how we could have made things safer.


And I'm pretty sure he won't try and store anything he does near anything we might eat.

This fella also seemed to be interested in building a clean-hood for several projects, including cultivating orchid seedlings! FYI, I like orchids, and they're a motherhacker to cultivate from seed, and having a clean hood to propagate them in greatly improves the chance of success. Ryan here could help FamiLAB propagate it's first special species of orchid!
I like colors.

Ryan

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Re: Ryan-Biohacking
« Reply #2 on: February 06, 2014, 11:23:52 AM »
Yeah, the peeps at familab know whatsup when it comes to electronics. I was discussing a centrifuge as well as some other things. I want to make a lab in a pizza box sort of thing and then do a kickstarter for DIY bio kits that has stuff like a pcr machine incubator centrifuge, tube holders, hotplate test tubes pitri dishes and maybe even some lab on a chip stuff.
 
ultimately I would like to do away with needing pipettes and make everything automated. Oh add a digital microscope/holographic digital superresolution/fluorescent scope to the pizza box idea.. Ask me more about that.
 
flow hoods are going to be crucial for a bio lab. I want to do a laminar flow hood with a giant filter and fan and perhaps even more precautions so viruses couldn't get through.
 
I'm putting together a sputter coater and maskless lithography system and it would be nice to have those in an environment where no small particles would land on any wafers.
 
this leads to the final goal I was mentioning above. I have an optoelectrowetting design I would like to use as a droplet processor that can integrate with microscope slide sized chips with various other applications. Everything from cell sorting and pcr to mass spec, chromatography, you name it...so I'm thinking we should all meet up some time to discuss further. When is good for you all??
 

nanolith

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Re: Ryan-Biohacking
« Reply #3 on: February 06, 2014, 01:25:33 PM »
I'm definitely interested in learning more about biohacking.

I have some experience growing cultures and even growing edible mushrooms from both spores and dikaryotic mycelia.  Back when I used to play with this, I put together a positive flow hood using a plastic enclosure, an old PC power supply, and a HEPA filter.  It wasn't perfect, but for transferring mycelia -- which have some resistance to bacterial infections -- it worked quite well.  I used a pressure canner as an autoclave, which did a fairly decent job.

Another possibility for biohacking, assuming that there are beer / wine brewers here, is keeping, purifying, and maintaining yeast lines.

whirlpool4

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Re: Ryan-Biohacking
« Reply #4 on: February 18, 2014, 02:24:40 PM »

nanolith

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Re: Ryan-Biohacking
« Reply #5 on: February 18, 2014, 02:50:52 PM »
\http://www.brewpi.com/ Arduino / Raspberry Pi-controlled brewing

Brew Pi.  Interesting.  I ended up putting together a networked PID controller using an Arduino and a (now defunct) wireless shield.  This was several years ago, however.  I also used this controller for modulating the speed of a fan that I mounted to my grill, for perfect temperature controlled smoking.

I wouldn't mind revisiting this project with a Beaglebone Black.

esperandus

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Re: Ryan-Biohacking
« Reply #6 on: February 18, 2014, 07:23:24 PM »
  DIY bio kits that has stuff like a pcr machine incubator centrifuge, tube holders, hotplate test tubes pitri dishes and maybe even some lab on a chip stuff.
 
ultimately I would like to do away with needing pipettes and make everything automated. Oh add a digital microscope/holographic digital superresolution/fluorescent scope to the pizza box idea.. Ask me more about that.
 


Something you might be interested in:
http://openpcr.org/


A friend of mine who worked in a lab at cornell had one of these at home. it seemed more of a toy than a tool to me, but honestly, these machines are so simple electronically and mechanically, that I see a huge potential for improvement on PCR machines (and many others) in the bio-world.


Honestly, I dont know why we dont already have completed automated robotic-pipette-using-growth centers that can more or less automate culture growth, isolation, PCR, etc. all in one go.

Ryan

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Re: Ryan-Biohacking
« Reply #7 on: February 18, 2014, 10:30:56 PM »
They have on chip pcr, pcr on cds, pcr in a million ways you can imagine them having pcr. I could use my armpits and two sticks and  do pcr.
 
as far as electrophoresis they have capillary electrophoresis and dielectrophoresis for separating DNA. When it comes to reading DNA there is a method that makes old sequencing techniques an expensive joke.

esperandus

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Re: Ryan-Biohacking
« Reply #8 on: February 19, 2014, 12:11:20 PM »
Quote
I could use my armpits and two sticks and  do pcr.


I demand a demonstration  8)

bentruck

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Re: Ryan-Biohacking
« Reply #9 on: February 19, 2014, 12:15:24 PM »
I would be willing to help with biohacking stuff. I'm always interested: just lacking funds.  :)
yes.

Ryan

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Re: Ryan-Biohacking
« Reply #10 on: February 19, 2014, 12:25:58 PM »
Challenge accepted!